Download Cellular Fatty Acid-binding Proteins by Robert K. Ockner (auth.), Jan F. C. Glatz, Ger J. Van Der PDF

By Robert K. Ockner (auth.), Jan F. C. Glatz, Ger J. Van Der Vusse (eds.)

Show description

Read or Download Cellular Fatty Acid-binding Proteins PDF

Similar nonfiction_10 books

Atlas of Human Anatomy: Volume I Osteology · Arthrology and Syndesmology Myology

AFTER ten years' training the 1st version of our Atlas of Human Anatomy used to be released among 1946 and 1951. Our adventure enabled us to enhance all of the next variants and the current one has additionally been completely revised and enlarged to permit the inclusion of extra instructive illus­ trations.

Chemically Induced Magnetic Polarization: Proceedings of the NATO Advanced Study Institute held at Sogesta, Urbino, Italy, April 17–30, 1977

Magnetic resonance has continually been capable of shock with its skill to show new phenomena. simply whilst it seems that to be coming into a quiet center age it bursts into task with a few new manifestation of its versatility. This occurred many years in the past, while observations on anomalous intensities have been checked out extra heavily, and the pursuit of factors and additional facts laid the rules of the themes taken care of during this quantity.

Pain Research: Methods and Protocols

The developments of clinical expertise, advancements in remedy, and elevated sufferers’ lifestyles span make soreness study and comparable drug improvement excessive priorities for either the examine neighborhood and pharmaceutical businesses. quick improvement of easy technological know-how examine instruments, comparable to concepts of flurometric labeling, genomic and proteomic excessive throughput screening, and genetically changed animals, promotes the speedy acceleration of discomfort examine to a degree permitting built-in investigations of soreness processing mechanisms on the unmarried mobile and/or molecule point, and in a spatially and temporally managed demeanour.

Molecular Dermatology: Methods and Protocols

The sustained pores and skin examine efforts during the last many years has ended in the buildup of an important number of info on epidermis constitution and body structure in addition to at the pathogenesis of cutaneous illnesses. In Molecular Dermatology: equipment and Protocols, best specialists within the box offer a set of cutting-edge trustworthy protocols overlaying a large spectrum of concepts and experimental versions, particular molecular assays and affliction types, in addition to overviews of diagnostic and study parts suitable to molecular dermatology.

Additional resources for Cellular Fatty Acid-binding Proteins

Example text

Molecular and Cellular Biochemistry 98: 27-33, 1990. © 1990 Kluwer Academic Publishers. Localization of liver fatty acid-binding protein and its mRNA in the liver and jejunum of rats: an immunohistochemical and in situ hybridization study Shoichi Isekil, Hisatake Kond02 , Masahiro HitomP and Teruo On0 4 1, 2 Department of Anatomy, Kanazawa University School of Medicine, Kanazawa, 920 Japan; 3,4 Department of Biochemistry, Niigata University School of Medicine, Niigata, 951 Japan Key words: liver fatty acid-binding protein, immunohistochemistry, in situ hybridization, liver, jejunum, rat Summary The localization of liver fatty acid-binding protein (L-FABP) and its mRNA in the liver and jejunum was examined in normal and 3-day-fasted rats by means of immunohistochemistry using a specific antibody to L-FABP and in situ hybridization using a synthetic oligonucleotide complementary to L-FABP mRNA as probe.

In situ hybridization The oligonucleotide probe was labeled by 3' -tailing to a specific activity of 1-2 X 108 cpmlJLg using [a35S]-dCTP (New England Nuclear). The hybridization procedure was essentially as described previously [17,18]. 015M sodium citrate), 1 X Denhardt's solution, 2% Sarkosyl,. 2), and then with a hybridization mixture containing 5 X 105 dpm of the labeled L-FABP probe. For the methodological control experiment, an excess amount (50 times that of the labeled probe) of the unlabeled template oligonucleotide complementary to the probe was added to the mixture.

1 % trifluoroacetic acid in acetonitrile were pooled. Peptides SP1 and SP2 were conjugated to Keyhole Limpet Hemocyanine (KLH) with maleimidobenzoyl-N-hydroxysuccinimide [25] using the cysteine-SH groups as described elsewhere [7]. The peptides SP3 and SP4 were coupled to KLH using a glutaraldehyde coupling method [26]. Control conjugates of the peptides with bovine serum albumin were produced using a carbodiimide coupling [27]. Isolation and purification of FABPc Human H-FABPc was isolated from tissue obtained within 12 hours post mortem.

Download PDF sample

Rated 4.32 of 5 – based on 37 votes