By Marcus Bantscheff (auth.), Gerard Drewes, Marcus Bantscheff (eds.)
The multidisciplinary technology of chemical proteomics stories how small molecules of artificial or usual starting place bind to proteins and modulate their functionality. In Chemical Proteomics: tools and Protocols, specialist researchers within the box offer key thoughts to enquire chemical proteomics concentrating on analytical techniques, how probes are generated, thoughts for the invention of small molecule pursuits and the probing of aim functionality, and small molecule ligand and drug discovery. Written within the hugely winning Methods in Molecular Biology™ sequence layout, chapters comprise introductions to their respective themes, lists of the required fabrics and reagents, step by step, easily reproducible laboratory protocols, and key pointers on troubleshooting and fending off identified pitfalls.
Authoritative and sensible, Chemical Proteomics : tools and Protocols seeks to supply methodologies that may give a contribution to a much wider software of chemical proteomics tools in biochemical and mobilephone organic laboratories.
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Extra info for Chemical Proteomics: Methods and Protocols
Continually agitate the solution for another 45 min. 5. Prepare a slurry of 4 mL Sephadex G-25 in water using a Sephadex to water ratio at 75:25. Carefully pour the slurry into a 3–5 mL plastic column. Wash the resin in the column with two column volumes. 6. Carefully apply the mixture from step 4 to the Sephadex G-25 column. 7. Insert the column into a long 15-mL plastic tube and centrifuge at 800 ´ g for 30 s. Unreacted small molecule compound is trapped in the Sephadex. Use the flow-through fraction (containing unreacted 5-FAM-TAT, 5-FAM-TAT reacted to LC-SMCC, and 5-FAM-TAT coupled to Bis-III through LC-SMCC) collected in the 15 mL tube for further purification.
R. (2002) The druggable genome. Nat. Rev. Drug Discov. 1, 727–730. 8. Brown, E. , Albers, M. , Shin, T. , Keith, C. , Lane, W. , and Schreiber, S. L. (1994) A mammalian protein targeted by G1-arresting rapamycin-receptor complex. Nature 369, 756–758. 9. Harding, M. , Uehling, D. , and Schreiber, S. L. (1989) A receptor for the immunosuppressant FK506 is a cis-trans peptidyl-prolyl isomerase. Nature 341, 758–760. 10. Huang, S. , Mishina, Y. , Michaud, G. , Wilson, C. , Kirschner, M. , Finan, P. , Tallarico, J.
Biochem. J. 404, 15–21. 29. , and Heck, A. J. (2010) Target profiling of a small library of phosphodiesterase 5 (PDE5) inhibitors using chemical proteomics. ChemMedChem. 5, 1927–1936. 30. Patricelli, M. , Szardenings, A. , Nomanbhoy, T. , and Kozarich, J. W. (2007) Functional interrogation of the kinome using nucleotide acyl phosphates. Biochemistry 46, 350–358. 31. , Fischer, J. , Baessler, O. , Schrey, A. , and Koester, H. (2010) GDPcapture compound – a novel tool for the profiling of GTPases in pro- and eukaryotes by capture compound mass spectrometry (CCMS).